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Embryo Vitrification

What Is It?

Vitrification is a cutting edge technology for cryopreservation (freezing) of embryos. In vitrification, the water molecules in an embryo are removed and replaced with a higher concentration of cryo-protectants. The embryos are then plunged directly into liquid nitrogen. This drastic freezing (-12000 ºC/minute) creates a glass transition temperature, commonly called a glass state, and the embryos are vitrified. This quick freezing reduces the chance of intracellular and extracellular ice crystals to be formed, thus decreasing the degeneration of cells upon thawing for embryo transfer. The embryos are then stored in liquid nitrogen at -196 ºC. At that extremely cold temperature, cellular activity is essentially brought to a halt, allowing the embryos to remain viable indefinitely.

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When Is It Indicated?

It is done in:

  • Patients at risk of Ovarian Hyper-Stimulation Syndrome (OHSS), so as to have an OHSS - Free Clinic.

  • Electively in a cycle, for better results due to better endometrial receptivity.

  • Excess healthy embryos in one cycle can be preserved for later cycles.

  • In cases of Pre-Implantation Genetic Screening and Diagnosis

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